The task of accurately separating, identifying, and quantitating amino acids in the research, development, and commercialization of food, feed, and biotherapeutic products is challenging. Common to these applications is the need for adequate sample hydrolysis, an important first step in the workflow for the analysis of bound amino acids, such as peptides and proteins. Hydrolysis allows for the analysis of the released amino acids, which can be separated using either ion-exchange or...
More DescriptionThe task of accurately separating, identifying, and quantitating amino acids in the research, development, and commercialization of food, feed, and biotherapeutic products is challenging. Common to these applications is the need for adequate sample hydrolysis, an important first step in the workflow for the analysis of bound amino acids, such as peptides and proteins. Hydrolysis allows for the analysis of the released amino acids, which can be separated using either ion-exchange or reversed-phase chromatography methods. Given the challenges of the sample preparation workflow, this guide provides an overview of the sample preparation considerations behind reliable, amino acid analysis of peptides and proteins. It is a starting point for those who perform amino acid analysis, as well as a resource for experienced analysts who are trying to resolve a related issue. The procedures detailed throughout the guide were obtained primarily from specific publications and official AOAC methods.