Viral Gene Techniques |
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| Author:
| Adolph, Kenneth W.
Author, Unknown |
| Editor:
| Adolph, Kenneth W. |
| Series title: | Methods in Molecular Genetics Ser. |
| ISBN: | 978-1-281-05921-5 |
| Publication Date: | Jan 2010 |
| Publisher: | Academic Press
|
| Book Format: | Ebook |
| List Price: | USD $87.54 |
Book Description:
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Viral Gene Techniques is a practical laboratory guide to current techniques of molecular biology and genetics. The volume is concerned with methods for the analysis of viral genes and chromosomes: DNA viruses and RNA viruses including HIV are discussed.
* Methods presented for ease of use and reasdy adaptation to new systems * Detailed experimental protocols included for: * Viral vectors - construction and use of DNA virus vectors (adenovirus, adeno-associated virus,...
More Description
Viral Gene Techniques is a practical laboratory guide to current techniques of molecular biology and genetics. The volume is concerned with methods for the analysis of viral genes and chromosomes: DNA viruses and RNA viruses including HIV are discussed.
* Methods presented for ease of use and reasdy adaptation to new systems
* Detailed experimental protocols included for:
* Viral vectors - construction and use of DNA virus vectors (adenovirus, adeno-associated virus, vaccinia virus, Epstein - Barr virus)
* DNA viruses - virus/host interactions, viral chromosomes, transcription regulation (viruses discussed include herpes simplex, hepatitis B, SV40, JC, Epstein-Barr, adenovirus)
* Human Immunodeficiency Virus / retroviruses -quantitation of HIV-1 virus stock and RNA, retrovirus reverse transcription / integration, retrovirus-mediated cell fusion, use as cell lineage markers
* RNA viruses - RNA virus assembly, analysis of RNA genomes, assays for RNA-binding proteins (viruses discussed include poliovirus, influenza virus, hepatitis delta virus)